At Headway, we are dedicated to providing healthcare professionals with reliable diagnostic tools that enhance patient care. Our 13C Urea Breath Test Kit is designed for the detection of Helicobacter pylori (H. pylori) infections, offering a non-invasive and effective method for diagnosis. In this article, we will outline the steps for using our 13C UBT test kit, ensuring that medical practitioners can implement it efficiently in their practice.

Step 1: Collecting the 0-Minute Exhaled Breath Sample

Preparation is essential before using the 13C UBT test kit for H.pylori infection. The subject undergoing testing should fast or refrain from eating for at least two hours prior to the test. This fasting period is crucial for obtaining an accurate baseline measurement of breath composition.

Once fasting is complete, the subject will slowly blow their exhaled breath into the exhalation bag provided in the 13C Urea Breath Test Kit, ensuring the bag is filled adequately. After filling the bag, it must be sealed properly. This sample serves as the 0-minute breath sample, establishing the baseline for subsequent measurements.

Step 2: Dosing the 13C Urea Capsule

After collecting the 0-minute breath sample, the next step involves dosing. The subject should swallow one urea [¹³C] capsule with 80-100 ml of cool water. It is important to advise the subject to take the capsule without chewing to ensure the urea is properly digested and absorbed. Following the ingestion of the capsule, the subject needs to wait for 30 minutes. This waiting period allows the urea to interact with any H. pylori present in the stomach, leading to the production of labeled carbon dioxide.

Step 3: Collecting the 30-Minute Exhaled Breath Sample

Thirty minutes after swallowing the 13C urea capsule, the subject returns to collect the second breath sample. They will again blow their exhaled breath into a different collection bag included in the 13C UBT test kit for H.pylori infection and seal it securely. This 30-minute breath sample will contain elevated levels of ¹³CO₂ if H. pylori is present, providing crucial data for analysis.

Step 4: Testing the Samples

The final step involves analyzing both the 0-minute and 30-minute exhaled breath samples for concentrations of ¹³CO₂ using the C13 UBT test detector. Results are expressed as δ‰ values. A subject is considered positive for H. pylori infection if the δ‰ is greater than or equal to 4.0. This simple yet effective testing process allows for accurate diagnosis and treatment planning.

Conclusion

In conclusion, the 13C Urea Breath Test Kit from Headway offers a straightforward and efficient method for detecting H. pylori infections. By following these steps, healthcare professionals can ensure reliable results and provide patients with the best possible care. We encourage medical institutions to utilize our 13C UBT test kit to enhance their diagnostic capabilities in identifying H. pylori infections. Together, we can improve gastrointestinal health outcomes and patient experiences in clinical settings.